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Effect of Enterococcus faecium on intestinal barrier integrity. ( A ) <t>MUC2</t> expression in the colon shows reduced staining intensity in the E. faecium group. ( B ) AB–PAS staining of goblet cells reveals atrophied and decreased goblet cell numbers in the E. faecium group. Images captured at 20× magnification; scale bar = 100 µm. ** p < 0.01, ns, not significant.
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Effect of Enterococcus faecium on intestinal barrier integrity. ( A ) <t>MUC2</t> expression in the colon shows reduced staining intensity in the E. faecium group. ( B ) AB–PAS staining of goblet cells reveals atrophied and decreased goblet cell numbers in the E. faecium group. Images captured at 20× magnification; scale bar = 100 µm. ** p < 0.01, ns, not significant.
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Effect of Enterococcus faecium on intestinal barrier integrity. ( A ) <t>MUC2</t> expression in the colon shows reduced staining intensity in the E. faecium group. ( B ) AB–PAS staining of goblet cells reveals atrophied and decreased goblet cell numbers in the E. faecium group. Images captured at 20× magnification; scale bar = 100 µm. ** p < 0.01, ns, not significant.
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Effect of Enterococcus faecium on intestinal barrier integrity. ( A ) MUC2 expression in the colon shows reduced staining intensity in the E. faecium group. ( B ) AB–PAS staining of goblet cells reveals atrophied and decreased goblet cell numbers in the E. faecium group. Images captured at 20× magnification; scale bar = 100 µm. ** p < 0.01, ns, not significant.

Journal: Pathogens

Article Title: Autism-Associated Gut Microbiota–Derived Enterococcus facium Modulates Gut–Brain Axis Function and Behavior in Mice

doi: 10.3390/pathogens14121191

Figure Lengend Snippet: Effect of Enterococcus faecium on intestinal barrier integrity. ( A ) MUC2 expression in the colon shows reduced staining intensity in the E. faecium group. ( B ) AB–PAS staining of goblet cells reveals atrophied and decreased goblet cell numbers in the E. faecium group. Images captured at 20× magnification; scale bar = 100 µm. ** p < 0.01, ns, not significant.

Article Snippet: Key antibodies, including MUC2, Occludin, Claudin 5, β-Actin, ZO-1, and Claudin 1 rabbit polyclonal antibodies, were sourced from Proteintech.

Techniques: Expressing, Staining